Genetically Encoded Voltage Indicators in Circulation Research

International Journal of Molecular Sciences

Atsushi Miyawaki¹  Andreas Müller²  Hidekazu Tsutsui³  Alessandra Moretti⁴  Daniel Sinnecker⁴  Peter Lipp⁵  Martin Oberhofer⁵  Lars Kaestner⁵  Elisabeth Kaiser⁵  Qinghai Tian⁵  Wenying Xian⁵  Sandra Ruppenthal⁵ 

[1] Cell Function Dynamics, Brain Science Institute, RIKEN, Wako 351-0192, Japan;Department of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg/Saar 66421, Germany;Department of Material Science, JAIST, Nomi, Ishikawa 923-1292, Japan;Medizinische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich 81675, Germany;Research Centre for Molecular Imaging and Screening, Institute for Molecular Cell Biology, Saarland University, Homburg/Saar 66421, Germany;

关键词: Genetically Encoded Voltage Indicators (GEVI);    membrane potential;    cardiomyocyte;    action potential;   

DOI  :  10.3390/ijms160921626

来源: DOAJ

【 摘 要 】

Membrane potentials display the cellular status of non-excitable cells and mediate communication between excitable cells via action potentials. The use of genetically encoded biosensors employing fluorescent proteins allows a non-invasive biocompatible way to read out the membrane potential in cardiac myocytes and other cells of the circulation system. Although the approaches to design such biosensors date back to the time when the first fluorescent-protein based Förster Resonance Energy Transfer (FRET) sensors were constructed, it took 15 years before reliable sensors became readily available. Here, we review different developments of genetically encoded membrane potential sensors. Furthermore, it is shown how such sensors can be used in pharmacological screening applications as well as in circulation related basic biomedical research. Potentials and limitations will be discussed and perspectives of possible future developments will be provided.

【 授权许可】

Unknown