Genetically Encoded Voltage Indicators in Circulation Research

International Journal of Molecular Sciences

Lars Kaestner³  Qinghai Tian³  Elisabeth Kaiser³  Wenying Xian³  Andreas Müller⁴  Martin Oberhofer³  Sandra Ruppenthal³  Daniel Sinnecker¹  Hidekazu Tsutsui²  Atsushi Miyawaki⁵  Alessandra Moretti¹  Peter Lipp³ 

[1] I. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich 81675, Germany; E-Mails:;Department of Material Science, JAIST, Nomi, Ishikawa 923-1292, Japan; E-Mail:;Research Centre for Molecular Imaging and Screening, Institute for Molecular Cell Biology, Saarland University, Homburg/Saar 66421, Germany; E-Mails:;Department of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg/Saar 66421, Germany; E-Mail:;Cell Function Dynamics, Brain Science Institute, RIKEN, Wako 351-0192, Japan; E-Mail:

关键词: Genetically Encoded Voltage Indicators (GEVI);    membrane potential;    cardiomyocyte;    action potential;   

DOI  :  10.3390/ijms160921626

来源: mdpi

【 摘 要 】

Membrane potentials display the cellular status of non-excitable cells and mediate communication between excitable cells via action potentials. The use of genetically encoded biosensors employing fluorescent proteins allows a non-invasive biocompatible way to read out the membrane potential in cardiac myocytes and other cells of the circulation system. Although the approaches to design such biosensors date back to the time when the first fluorescent-protein based Förster Resonance Energy Transfer (FRET) sensors were constructed, it took 15 years before reliable sensors became readily available. Here, we review different developments of genetically encoded membrane potential sensors. Furthermore, it is shown how such sensors can be used in pharmacological screening applications as well as in circulation related basic biomedical research. Potentials and limitations will be discussed and perspectives of possible future developments will be provided.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.