Increased Preoperative Plasma Level of Microbial 16S rDNA Translocation Is Associated With Relapse After Prostatectomy in Prostate Cancer Patients

Frontiers in Oncology

Zejun Zhou²  Baoli Zhu³  David P. Turner⁴  Tongwen Ou⁵  Michael Lilly⁶  Wei Jiang⁷ 

[1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Beijing Key Laboratory of Antimicrobial Resistance and Pathogen Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, United States;Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, China;Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, United States;Department of Urology, Capital Medical University Affiliated XuanWu Hospital, Beijing, China;Division of Hematology and Oncology, Department of Medicine, Medical University of South Carolina, Charleston, SC, United States;Division of Infectious Diseases, Department of Medicine, Medical University of South Carolina, Charleston, SC, United States;Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, China;

关键词: prostate cancer;    microbial translocation;    prostatectomy;    relapse;    prostate-specific antigen;   

DOI  :  10.3389/fonc.2019.01532

来源: DOAJ

【 摘 要 】

Background: The environmental factors for promoting prostate cancer (PCa) recurrence remain unknown.Methods: A retrospective cross-sectional study was conducted in healthy men (n = 12) and PCa patients undergoing prostatectomy (n = 27). Plasma preoperative level of total cell-free bacterial 16S rDNA, a marker of microbial translocation, was evaluated by qPCR. Plasma levels of prostate-specific antigen (PSA) were evaluated by ELISA.Results: Similar degrees of microbial translocation were found in healthy men and patients. However, the levels of microbial 16S rDNA were increased in patients with cancer relapse (n = 10) compared to patients without relapse (n = 17) after prostatectomy. Furthermore, the levels of microbial 16S rDNA were marginally increased in patients with pT3 or pT4 tumors compared to those with pT 2 or less. The levels of microbial 16S rDNA tended to increase in patients with higher pathologic tumor stage, Gleason score, and margin and lymph node involvements; but these differences did not reach significance.Conclusion: The plasma 16S rDNA levels increased in patients with PCa who have biochemical recurrence and 16S rDNA levels were higher in patients with higher-grade PCa.

【 授权许可】

Unknown