Lignosulfonate Rapidly Inactivates Human Immunodeficiency and Herpes Simplex Viruses

Medicines

Yuji Kikkawa¹  Hiromu Takemura²  Daisuke Asai²  Takuro Koshikawa²  Megumi Inomata³  Kunihiko Fukuchi⁴  Taisei Kanamoto⁵  Hiroshi Sakagami⁶  Hikaru Aimi⁷ 

[1] Chemical Sales Department II, Chemical Sales Division, Nippon Paper Industries Co., Ltd., 4-6 Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan;Department of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae, Kawasaki 216-8511, Japan;Division of Microbiology, Meikai University School of Dentistry, Keyakidai 1-1, Sakado, Saitama 350-0283, Japan;Graduate School of Health Sciences, Showa University, Hatanodai 1-5-8, Shinagawa, Tokyo 142-8555, Japan;Laboratory of Microbiology, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan;Research Institute of Odontology (M-RIO), Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, Japan;Research Laboratory, Research and Development Division, Nippon Paper Industries Co., Ltd., 5-21-1 Oji, Kita-ku, Tokyo 114-0002, Japan;

关键词: lignosulfonates;    anti-HIV;    anti-HSV;    quick inactivation of virus;    water solubility;    MTT assay;   

DOI  :  10.3390/medicines8100056

来源: DOAJ

【 摘 要 】

Background: Very few studies of the antiviral potential of lignosulfonates have been published. With the aim of oral application, among various groups of natural products, the relative antiviral potency of lignosulfonate and its ability to rapidly inactivate viruses were investigated. Methods: As target cells, MT-4 cells in suspension and attached Vero cells were used for infections with human immunodeficiency virus (HIV) and human herpes simplex type-1 virus (HSV). Mock- or virus-infected cells were incubated for 3–5 days with various concentrations of test samples, and the viable cell number was determined with the MTT method. For the shorter exposure experiments, higher titers of HIV or HSV were exposed to test samples for 10 or 3 min, diluted to a normal multiplicity of infection (MOI), and applied to the cells. Antiviral activity was quantified by using the chemotherapy index. Results: In the long-exposure system, lignosulfonates showed comparable anti-HIV activity with those of AZT, ddC, and sulfated polysaccharides, and it exceeded those of hundreds of tannins and flavonoids. When the exposure time was shortened, the chemotherapeutic index of the lignosulfonates for HIV was increased 27-fold. At a physiological pH, lignosulfonate showed higher anti-HIV activity than commercial alkali-lignin, dealkali-lignin, and humic acid, possibly due to the higher solubility and purity. Conclusions: With their rapid virus-inactivation capabilities, lignosulfonates may be useful for the prevention or treatment of virally induced oral diseases.

【 授权许可】

Unknown