Developmentally Driven Changes in Adipogenesis in Different Fat Depots Are Related to Obesity

Frontiers in Endocrinology

Yvonne Böttcher¹  Stephanie Kehr²  Peter F. Stadler⁵  Peter Kovacs⁸  Matthias Blüher⁸  Luise Müller⁸  Michael Stumvoll⁸  Jana Breitfeld^1,10 

[1] 0Department of Clinical Molecular Biology, Akershus Universitetssykehus, Lørenskog, Norway;Bioinformatics Group, Department of Computer Science and Interdisciplinary Center for Bioinformatics, University of Leipzig, Leipzig, Germany;Facultad de Ciencias, Universidad National de Colombia, Bogotá, Colombia;German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany;Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria;Institute of Clinical Medicine, University of Oslo, Oslo, Norway;Max Planck Institute for Mathematics in the Sciences, Leipzig, Germany;Medical Department III—Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, Leipzig, Germany;Santa Fe Institute, Santa Fe, NM, United States;University of Leipzig Medical Center, IFB Adiposity Diseases, University of Leipzig, Leipzig, Germany;

关键词: adipogenesis;    transcriptome;    adipocyte;    mRNA;    gene expression;    TTCA;   

DOI  :  10.3389/fendo.2020.00138

来源: DOAJ

【 摘 要 】

Subcutaneous (sc) and visceral (vis) adipose tissue (AT) contribute to the variability in pathophysiological consequences of obesity and adverse fat distribution. To gain insights into the molecular mechanisms distinguishing vis and sc fat, we compared the transcriptome during differentiation of immortalized adipocytes from murine epididymal (epi) and inguinal (ing) AT. RNA was extracted on different days of adipogenesis (−2, 0, 2, 4, 6, 8) and analyzed using Clariom™ D mouse assays (Affymetrix) covering >214,900 transcripts in >66,100 genes. Transcript Time Course Analysis revealed 137 differentially expressed genes. The top genes with most divergent expression dynamics included developmental genes like Alx1, Lhx8, Irx1/2, Hoxc10, Hoxa5/10, and Tbx5/15. According to pathway analysis the majority of the genes were enriched in pathways related to AT development. Finally, in paired samples of human vis and sc AT (N = 63), several of these genes exhibited depot-specific variability in expression which correlated closely with body mass index and/or waist-to-hip ratio. In conclusion, intrinsically programmed differences in gene expression patterns during adipogenesis suggest that fat depot specific regulation of adipogenesis contributes to individual risk of obesity.

【 授权许可】

Unknown