Andrographolide induces apoptosis via down-regulation of glyoxalase 1 and HMG-CoA reductase in HL-60 cells

Journal of Functional Foods

Robert J. Doerksen¹  Mei-Li Wu²  Tzou-Chi Huang²  Chun-Chia Chen²  Chi-Tang Ho³ 

[1] Department of BioMolecular Sciences and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS 38677-1848, USA;Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA;

关键词: Andrographolide;    Glyoxalase 1;    Glutamate-cysteine ligase catalytic subunit;    HMG-CoA reductase;    Apoptosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Andrographolide (Andro) is the major active component of the traditional herbal bitter tea Andrographis paniculata. We tested the hypothesis that Andro exerted its antitumor activities through its effects on three key survival enzymes, glyoxalase 1 (GLO1), glutamate-cysteine ligase catalytic subunit (GCLC) and HMG-CoA reductase (HMGCR), leading to cancer cell apoptosis. In human leukemia HL-60 cells Andro could reduce cholesterol levels, accompanied with down-regulation of Ras translocation to the membrane and downstream phosphorylation of Akt and ERK. Based on results obtained using specific inhibitors, LY294002 (PI3K inhibitor), U0126 (MEK1/2 inhibitor), and JSH-23 (NF-κB activation inhibitor), we proposed that the apoptotic effects of Andro in HL-60 cells arose because of interactions with the Ras/Akt/NF-κB/GLO1 and Ras/Raf/ERK/NF-κB/GLO1 pathways. Andro has a potential to be used as a functional food ingredient to prevent and manage inflammatory diseases including cancers.

【 授权许可】

Unknown