Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Biosensors

Marco Rasponi¹  Cecilia Palma¹  Erika Ferrari¹  Paola Occhetta¹  Simone Vesentini¹ 

[1] Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133 Milano, Italy;

关键词: organs-on-chip;    microphysiological systems;    microfluidics;    microfabrication;    biosensors;    electrodes;   

DOI  :  10.3390/bios10090110

来源: DOAJ

【 摘 要 】

Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

【 授权许可】

Unknown