Sensing with Nanopores and Aptamers: A Way Forward

Sensors

Aurélie Bouchet-Spinelli¹  Arnaud Buhot¹  Camille Raillon¹  Lucile Reynaud¹ 

[1] Univ. Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, France;

关键词: nanopores;    nanopipettes;    nanochannels;    aptamers;    biological pores;    translocation;   

DOI  :  10.3390/s20164495

来源: DOAJ

【 摘 要 】

In the 90s, the development of a novel single molecule technique based on nanopore sensing emerged. Preliminary improvements were based on the molecular or biological engineering of protein nanopores along with the use of nanotechnologies developed in the context of microelectronics. Since the last decade, the convergence between those two worlds has allowed for biomimetic approaches. In this respect, the combination of nanopores with aptamers, single-stranded oligonucleotides specifically selected towards molecular or cellular targets from an in vitro method, gained a lot of interest with potential applications for the single molecule detection and recognition in various domains like health, environment or security. The recent developments performed by combining nanopores and aptamers are highlighted in this review and some perspectives are drawn.

【 授权许可】

Unknown