Metabolomic and transcriptomic profiling of adult mice and larval zebrafish leptin mutants reveal a common pattern of changes in metabolites and signaling pathways

Cell & Bioscience

Mariëlle C. Haks¹  Jörg Matysik²  Yi Ding³  Herman P. Spaink³  Natalia Nowik³  Gabriel Forn-Cuní³  Junling He³  A. Alia⁴  Muhamed N. H. Eeza⁴  Amy C. Harms⁵  Thomas Hankemeier⁵ 

[1] Department of Infectious Diseases, Leiden University Medical Center;Institute of Analytical Chemistry, University of Leipzig;Institute of Biology, Leiden University;Institute of Medical Physics and Biophysics, University of Leipzig;Leiden Academic Centre for Drug Research, Leiden University;

关键词: Ob/ob mice;    Leptin mutant zebrafish;    Diabetes;    Metabolomics;    Transcriptomics;    Wasting syndrome;   

DOI  :  10.1186/s13578-021-00642-0

来源: DOAJ

【 摘 要 】

Abstract Background Leptin plays a critical role in the regulation of metabolic homeostasis. However, the molecular mechanism and cross talks between leptin and metabolic pathways leading to metabolic homeostasis across different species are not clear. This study aims to explore the effects of leptin in mice and zebrafish larvae by integration of metabolomics and transcriptomics. Different metabolomic approaches including mass spectrometry, nuclear magnetic resonance (NMR) and high-resolution magic-angle-spinning NMR spectrometry were used to investigate the metabolic changes caused by leptin deficiency in mutant ob/ob adult mice and lepb −/− zebrafish larvae. For transcriptome studies, deep RNA sequencing was used. Results Thirteen metabolites were identified as common biomarkers discriminating ob/ob mice and lepb −/− zebrafish larvae from their respective wild type controls: alanine, citrulline, ethanolamine, glutamine, glycine, histidine, isoleucine, leucine, methionine, phenylalanine, putrescine, serine and threonine. Moreover, we also observed that glucose and lipid levels were increased in lepb −/− zebrafish larvae compared to the lepb +/+ group. Deep sequencing showed that many genes involved in proteolysis and arachidonic acid metabolism were dysregulated in ob/ob mice heads and lepb mutant zebrafish larvae compared to their wild type controls, respectively. Conclusions Leptin deficiency leads to highly similar metabolic alterations in metabolites in both mice and zebrafish larvae. These metabolic changes show similar features as observed during progression of tuberculosis in human patients, mice and zebrafish larvae. In addition, by studying the transcriptome, we found similar changes in gene regulation related to proteolysis and arachidonic acid metabolism in these two different in vivo models.

【 授权许可】

Unknown