Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage

Frontiers in Molecular Neuroscience

Mathias Hafner¹  Norbert Gretz¹  Matteo Rigon²  Sarah Janice Hörner²  Tatjana Straka²  Karen Bieback³  Rüdiger Rudolf⁴ 

[1] Institute of Medical Technology, Medical Faculty Mannheim, Mannheim University of Applied Sciences, Mannheim, Germany;Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany;Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany;Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany;Medical Research Center, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany;

关键词: glycerol;    skeletal muscle;    NMJ;    ASC;    regeneration;   

DOI  :  10.3389/fnmol.2020.00107

来源: DOAJ

【 摘 要 】

Amongst other approaches, adipose-derived stromal cells (ASCs) have recently been tested with respect to their regenerative capacity for treatment of neuromuscular disorders. While beneficial effects of ASCs on muscle recovery were observed previously, their impact on regeneration of neuromuscular junctions (NMJs) is unclear. Here, we used a murine glycerol damage model to study disruption and regeneration of NMJs and to evaluate the effects of systemic application of ASCs on muscle and NMJ recovery. In mice that were not treated with ASCs, a differential response of NMJ pre- and post-synapses to glycerol-induced damage was observed. While post-synapses were still present in regions that were necrotic and lacking actin and dystrophin, pre-synapses disappeared soon in those affected areas. Partial regeneration of NMJs occurred within 11 days after damage. ASC treatment slightly enhanced NMJ recovery and reduced the loss of presynaptic sites, but also led to a late phase of muscle necrosis and fibrosis. In summary, the results suggest a differential sensitivity of NMJ pre- and post-synapses to glycerol-induced muscle damage and that the use of ASC for the treatment of neuromuscular disorders needs further careful evaluation.

【 授权许可】

Unknown