miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma

FEBS Open Bio

Qian Deng¹  Yazhuo Jiang¹  Yongyi Cheng¹  Yi Sun¹  Zheng Yang²  Lei Li²  Liang Liang² 

[1] Department of Urology Shaanxi Provincial People's Hospital Xi'an China;Department of Urology The First Affiliated Hospital of Xi'an Jiaotong University China;

关键词: ATG5;    autophagy;    miR‐30d‐5p;    proliferation;    renal cell carcinoma;   

DOI  :  10.1002/2211-5463.13025

来源: DOAJ

【 摘 要 】

Previous reports have shown that miR‐30d‐5p functions as a tumor suppressor in prostate cancer and gallbladder carcinoma, but its role in renal cell carcinoma (RCC) remains elusive. This study was designed to explore the functional role of miR‐30d‐5p in proliferation and autophagy of RCC. Our results show that miR‐30d‐5p is significantly down‐regulated in RCC tissues compared with normal tissues. miR‐30d‐5p overexpression suppressed cell proliferation, cell‐cycle G1/S transition and autophagy, but promoted apoptosis in RCC cell lines (786‐O and ACHN). Intriguingly, autophagy‐related gene 5 (ATG5) was directly targeted by miR‐30d‐5p, as shown using luciferase reporter assay and biotin‐avidin pull‐down assay. Moreover, overexpression of ATG5 attenuated the inhibitory effect of miR‐30d‐5p on proliferation and autophagy in 786‐O cells. These results suggest that miR‐30d‐5p suppresses proliferation and autophagy in RCC cells by targeting ATG5, and this pathway may be a suitable basis for the design of novel cancer therapeutics.

【 授权许可】

Unknown