Effect of digital hypothermia on lamellar inflammatory signaling in the euglycemic hyperinsulinemic clamp laminitis model

Journal of Veterinary Internal Medicine

Simon M. Stokes¹  François‐René Bertin¹  Carlos E. Medina‐Torres¹  Andrew W. vanEps¹  Teresa A. Burns²  James K. Belknap²  Mauria R. Watts²  Darko Stefanovski³ 

[1] Australian Equine Laminitis Research Unit, School of Veterinary Science The University of Queensland Gatton Queensland Australia;Department of Veterinary Clinical Sciences The Ohio State University College of Veterinary Medicine Columbus Ohio USA;New Bolton Center, Department of Clinical Studies, School of Veterinary Medicine University of Pennsylvania Kennett Square Pennsylvania USA;

关键词: endocrinopathic;    horse;    hypothermia;    inflammation;    insulin;   

DOI  :  10.1111/jvim.15835

来源: DOAJ

【 摘 要 】

Abstract Background Continuous digital hypothermia (CDH) prevents lamellar failure in the euglycemic hyperinsulinemic clamp (EHC) model of laminitis, but the protective mechanisms are unclear. Hypothesis/Objectives To determine if CDH inhibits lamellar inflammatory signaling in the EHC model of laminitis. Animals Eight Standardbred horses. Methods Prospective experimental study. Horses underwent an EHC, with 1 forelimb treated with CDH and the other kept at ambient temperature (AMB). Horses were euthanized 48 hours after initiation of the EHC and lamellar tissue was analyzed via polymerase chain reaction (pro‐inflammatory cytokine and chemokine genes—CXCL1, CXCL6, CXCL8, IL‐6, MCP‐1, MCP‐2, IL‐1β, IL‐11, cyclooxygenase 1 and 2, tumour necrosis factor‐alpha [TNF‐α], E‐selectin, and intercellular adhesion molecule‐1 [ICAM‐1]) and immunoblotting (phosphorylated and total signal transducer and activator of transcription 1 [STAT1] and STAT3). Results Compared to AMB, lamellar messenger ribonucleic acid (mRNA) concentrations of CXCL6 (P =.02), CXCL8 (P = .008), IL‐6 (P = .008), IL‐1β (P = .008), IL‐11 (P = .008), and cyclooxygenase‐2 (P = .008) were decreased in CDH. Cyclooxygenase‐1 (P = .008) was increased in CDH, while CXCL1 (P = .15), MCP‐1 (P = .05), MCP‐2 (P = .46), TNF‐α (P = .05), E‐selectin (P = .15), and ICAM‐1 (P = .15) mRNA were not significantly different. Compared to AMB, lamellar concentration of total STAT3 protein was decreased in CDH (P < .001), but there was no change in phosphorylated STAT3 (P‐STAT3 [S727] P = .19; P‐STAT3 [Y705] P = .05). There was no change in lamellar concentrations of total STAT1 (P = .75) or phosphorylated STAT1 (P‐STAT1 [S727], P = .25; P‐STAT1 [Y701], P = .64). Conclusions and Clinical Importance These data add further support for the use of CDH as a first aid treatment for severe acute laminitis associated with hyperinsulinemia in horses.

【 授权许可】

Unknown