One health pathogen surveillance demonstrated the dissemination of gut pathogens within the two coastal regions associated with intensive farming

Gut Pathogens

Yixiang Zhang¹  Tom Defoirdt²  Qian Yang²  Baocheng Qu³  Qingyao Wang³  Songzhe Fu³ 

[1] CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences;Center for Microbial Ecology and Technology (CMET), Ghent University;College of Marine Science and Environment, Dalian Ocean University;

关键词: Vibrio parahaemolyticus;    Vibrio vulnificus;    Wetlands;    One health;    Shrimp farming;   

DOI  :  10.1186/s13099-021-00442-4

来源: DOAJ

【 摘 要 】

Abstract Background Intensive aquaculture farming has caused significant degradation of coastal wetlands and has been proposed as a reservoir for pathogenic Vibrio spp. Results Gut pathogens including Vibrio spp., Salmonella spp., and Klebsiella spp. were isolated from bird feces, shrimp and wetland water in two typical coastal regions of China in 2015 and 2017 and were subsequently subjected to whole-genome sequencing. Meanwhile, local patient isolates were also selected to confirm the epidemiological links. Bacterial community composition analyses of the sediments that were sampled in 2015 and 2017 were conducted by the hypervariable region 4 of the 16S rRNA gene. Together with the local clinical isolates, we observed highly related Vibrio isolates from waterbirds, wetlands and shrimp. Phylogenetic genome comparisons also demonstrated that sequence types ST3 and ST2414 Vibrio parahaemolyticus isolates obtained from aquatic animals were clonally related to patient isolates. Likewise, three Salmonella typhimurium isolates were also genomically related to one clinical strain. The results showed that farming activities significantly altered the community composition and resulted in the emergence of several pathogens, including Acinetobacter, Mycobacterium and Legionella. Conclusions In conclusion, our results demonstrated that intensive shrimp farming in wetlands has two devastating impacts: pathogen dissemination from aquatic animals into migratory birds and transmission of foodborne pathogens into local communities.

【 授权许可】

Unknown