Applications of Atomic Force Microscopy in HIV-1 Research

Viruses

Itay Rousso¹  Akshay Deshpande¹ 

[1] Department of Physiology and Cell Biology, Ben-Gurion University of the Negev, Beer Sheva 8443944, Israel;

关键词: atomic force microscopy;    HIV-1;    mechanical properties;    imaging;   

DOI  :  10.3390/v14030648

来源: DOAJ

【 摘 要 】

Obtaining an understanding of the mechanism underlying the interrelations between the structure and function of HIV-1 is of pivotal importance. In previous decades, this mechanism was addressed extensively in a variety of studies using conventional approaches. More recently, atomic force microscopy, which is a relatively new technique with unique capabilities, has been utilized to study HIV-1 biology. Atomic force microscopy can generate high-resolution images at the nanometer-scale and analyze the mechanical properties of individual HIV-1 virions, virus components (e.g., capsids), and infected live cells under near-physiological environments. This review describes the working principles and various imaging and analysis modes of atomic force microscopy, and elaborates on its distinctive contributions to HIV-1 research in areas such as mechanobiology and the physics of infection.

【 授权许可】

Unknown