Phenotypic responses and potential genetic mechanism of lepidopteran insects under exposure to graphene oxide

Ecotoxicology and Environmental Safety

Tiantao Zhang¹  Kanglai He²  Zhenying Wang³  Haicui Xie⁴  Xiuping Wang⁴  Xiaoduo Gao⁴  Dapeng Jing⁴ 

[1] Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, PR China;Corresponding author.;College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, PR China;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China;

关键词: Graphene oxide;    Asian corn borer;    Promote the growth;    Trypsin-like serine protease;   

DOI  :  

来源: DOAJ

【 摘 要 】

Clarification of the interactions between engineered nanomaterials and multiple generations of insects is crucial to understanding the impact of nanotechnology on the environment and agriculture, particularly in toxicity management, pest management and genetic engineering. To date, there has been very limited information about nanoparticle-insect interactions at the genetic and proteomic levels. Here, we examined the phenotypic responses and potential mechanism of a lepidopteran insect Asian corn borer (ACB) to graphene oxide (GO). It was demonstrated that GO could significantly promote the growth of ACB. The transcriptomic and proteomic results consistently verified that GO might activate trypsin-like serine protease, glutathione S-transferase, heat shock protein and glycosyltransferase to further influence the development of ACB. RNA interference results indicated that the trypsin gene was one of the critical genes to accelerate the growth of ACB fed with GO diet. Moreover, physiological analysis showed potential alterations of the expression levels of genes and proteins, and more cholesterol (CE), triacylglycerides (TG) and lipids were accumulated in GO-exposed ACB. Our findings may help to reveal the phenotypic, physiological and genetic responses of insects under exposure to nanomaterials and to assess the environmental risks of other nanomaterials.

【 授权许可】

Unknown