Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS

Frontiers in Pharmacology

Salma Malik¹  Nanda Gamad¹  Kapil Suchal¹  Jagriti Bhatia¹  Rajiv Kumar Malhotra¹  Dharamvir S. Arya¹  Tapas C. Nag²  Shreesh Ojha³  Sameer Goyal⁴ 

[1]Cardiovascular Research Laboratory, Department of Pharmacology, All India Institute of Medical SciencesNew Delhi, India

[2]Department of Anatomy, All India Institute of Medical SciencesNew Delhi, India

[3]Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates UniversityAbu Dhabi, UAE

[4]Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and ResearchShirpur, India

关键词: apoptosis;    myocardial ischemia–reperfusion injury;    inflammation;    oxidative stress;    seabuckthorn;    lehberry;   

DOI  :  10.3389/fphar.2016.00155

来源: DOAJ

【 摘 要 】

Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt–eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression.

【 授权许可】

Unknown