Quantitative and Statistical Study of the Dynamics of Clathrin-Dependent and -Independent Endocytosis Reveal a Differential Role of EndophilinA2

Cell Reports

Cyril Basquin¹  Laetitia Bertot²  Nathalie Sauvonnet²  Alexandre Grassart²  Giacomo Nardi³  Thibault Lagache³  Jean-Christophe Olivo-Marin³ 

[1] Institut Jacques Monod, Equipe de Polarité Cellulaire dans le Développement et l’évolution, CNRS UMR 7592. 15, rue Hélène Brion, 75205 Paris Cedex 13, France;Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, INSERM U1202. 28, rue du Docteur Roux, 75015 Paris, France;Unité d’Analyse d’Images Biologiques, Institut Pasteur, CNRS UMR3691. 25, rue du Docteur Roux, 75015 Paris, France;

关键词: interleukin-2 receptor;    internalization;    cytokine;    FEME;    BAR;    vesicle fission;   

DOI  :  10.1016/j.celrep.2018.01.039

来源: DOAJ

【 摘 要 】

Summary: Eukaryotic cells internalize cargos specifically through clathrin-mediated endocytosis (CME) or clathrin-independent endocytosis (CIE). EndophilinA2 was shown as preferentially implicated in CIE, although initially involved in CME. Here, we investigated the native interplay of endophilinA2 and dynamin2 during CME as compared to CIE. We developed an unbiased integrative approach based on genome engineering, robust tracking methodology, and advanced analytics. We statistically identified CME and CIE subpopulations corresponding to abortive, active, and static endocytic events. Depletion of dynamin2 strongly affected active CME and CIE events, whereas the absence of endophilinA2 impacted only CIE. Accordingly, we demonstrated that endophilinA2 is needed for dynamin2 recruitment during CIE, but not in CME. Despite these differences, endophilinA2 and dynamin2 acted at the latest stage of endocytosis within a similar stoichiometry in both mechanisms. Thus, we propose a conserved function of dynamin2 and endophilinA2 in vesicle scission, but a differential regulation of their recruitment during CME and CIE.

【 授权许可】

Unknown