Cell type‐specific tyrosine phosphorylation of IL‐2 receptor β chain in response to IL‐2

FEBS Letters

Kurahayashi, Yumiyo¹  Nakamura, Masataka¹  Takeshita, Toshikazu¹  Sugamura, Kazuo¹  Beckers, Thomas²  Kumaki, Satoru¹  Asao, Hironobu¹  Engels, Joachim W.² 

[1] Department of Microbiology, Tohoku University, School of Medicine, 2-1 Seiryo-machi, Aoba-Ku, Sendai 980 Japan;Institut für Organische Chemie, Universität Frankfurt, Niederurseler Hang, 6000 Frankfurt/Main 50, Germany

关键词: Interleukin-2 receptor β chain;    Tyrosine kinase;    IL-2R;    interleukin-2 receptor;    FCS;    fetal calf serum;    BSA;    bovine serum albumin;    mAb;    monoclonal antibody;    2D PAGE;    two-dimensional polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)81137-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Functional activities of the IL-2 receptor (IL-2R) β chain exogenously expressed on lymphoid and non-lymphoid cells were examined in terms of phosphorylation of IL-2Rβ and cell growth. Lymphoid MOLT-4 and its transfectants expressing IL-2Rβ either alone or with IL-2R α chain were found to be rapidly phosphorylated predominantly at tyrosine residues of IL-2Rβ and to be affected in their growth in an IL-2-dependent manner. In contrast, IL-2 induced neither phosphorylation of IL-2Rβ nor cell growth in non-lymphoid transfectants derived from COS7, HeLa and L929, even though they acquired the IL-2 binding ability when coexpressed as IL-2Rβ and IL-2Rα. These results suggest that IL-2 induces activation of a tyrosine kinase possibly associated with IL-2Rβ in a cell type-specific manner.

【 授权许可】

Unknown