Amphiphilic and Perfluorinated Poly(3-Hydroxyalkanoate) Nanocapsules for 19F Magnetic Resonance Imaging

Bioengineering

Estelle Renard¹  Valérie Langlois¹  Marion Le Gal²  Christelle Simon-Colin²  Benoit Larrat³ 

[1] ICMPE, CNRS, University Paris Est Creteil, F-94010 Creteil, France;Laboratoire de Microbiologie des Environnements Extrêmes, CNRS, Ifremer, University Brest, F-29280 Plouzané, France;Université Paris-Saclay, CEA, CNRS, NeuroSpin, 91191 Gif-sur-Yvette, France;

关键词: polyhydroxyalkanoates;    mcl-PHA;    nanocapsules;    fluorinated polymer;    amphiphilic copolymer;   

DOI  :  10.3390/bioengineering8090121

来源: DOAJ

【 摘 要 】

Nanoparticles have recently emerged as valuable tools in biomedical imaging techniques. Here PEGylated and fluorinated nanocapsules based on poly(3-hydroxyalkanoate) containing a liquid core of perfluorooctyl bromide PFOB were formulated by an emulsion-evaporation process as potential ¹⁹F MRI imaging agents. Unsaturated poly(hydroxyalkanoate), PHAU, was produced by marine bacteria using coprah oil and undecenoic acid as substrates. PHA-g-(F; PEG) was prepared by two successive controlled thiol-ene reactions from PHAU with firstly three fluorinated thiols having from 3 up to 17 fluorine atoms and secondly with PEG-SH. The resulting PHA-g-(F; PEG)-based PFOB nanocapsules, with a diameter close to 250–300 nm, are shown to be visible in ¹⁹F MRI with an acquisition time of 15 min. The results showed that PFOB-nanocapsules based on PHA-g-(F; PEG) have the potential to be used as novel contrast agents for ¹⁹F MRI.

【 授权许可】

Unknown