Glycosphingolipids of the model fungus Aspergillus nidulans

Journal of Lipid Research

Rebecca Lindsey¹  Steven B. Levery²  Matthew Fuller²  Chaeho Park³  Richard Jennemann³  Beau Bennion⁴  Michelle Momany⁴ 

[1] Abteilung für Zelluläre und Molekuläre Pathologie, Deutsches Krebsforschungszentrum-Heidelberg, Im Neuenheimer Feld 280, D69120, Heidelberg, Germany;Department of Plant Biology, University of Georgia, Athens, GA 30602-7229;The Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602-7229;Department of Chemistry, University of New Hampshire, Durham, NH 03824-3598;

关键词: glycolipid;    mass spectrometry;    tandem mass spectrometry;    collision-induced dissociation;    electrospray ionization;    NMR spectroscopy;   

DOI  :  

来源: DOAJ

【 摘 要 】

Aspergillus nidulans is a well-established nonpathogenic laboratory model for the opportunistic mycopathogen, A. fumigatus. Some recent studies have focused on possible functional roles of glycosphingolipids (GSLs) in these fungi. It has been demonstrated that biosynthesis of glycosylinositol phosphorylceramides (GIPCs) is required for normal cell cycle progression and polarized growth in A. nidulans (Cheng, J., T.-S. Park, A. S. Fischl, and X. S. Ye. 2001. Mol. Cell Biol. 21: 6198–6209); however, the structures of A. nidulans GIPCs were not addressed in that study, nor were the functional significance of individual structural variants and the downstream steps in their biosynthesis. To initiate such studies, acidic GSL components (designated An-2, -3, and -5) were isolated from A. nidulans and subjected to structural characterization by a combination of one-dimensional (1-D) and 2-D NMR spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), ESI-MS/collision-induced decomposition-MS (MS/CID-MS), ESI-pseudo-[CID-MS]2, and gas chromatography-MS methods.All three were determined to be GIPCs, with mannose as the only monosaccharide present in the headgroup glycans; An-2 and An-3 were identified as di- and trimannosyl inositol phosphorylceramides (IPCs) with the structures Manα1→3Manα1→2Ins1-P-1Cer and Manα1→3(Manα1→6)Manα1→2Ins1-P-1Cer, respectively (where Ins = myo-inositol, P = phosphodiester, and Cer = ceramide). An-5 was partially characterized, and is proposed to be a pentamannosyl IPC, based on the trimannosyl core structure of An-3.

【 授权许可】

Unknown