Preparation of BMP-2/PDA-BCP Bioceramic Scaffold by DLP 3D Printing and its Ability for Inducing Continuous Bone Formation

Frontiers in Bioengineering and Biotechnology

Gang Zhang¹  Yinghui Tan¹  Yujiang Fan²  Changchun Zhou²  Boqing Zhang²  Weidong Tian³  Fangjun Huo³  Li Xie³  Xi Liang³  Ziyang Yang³ 

[1] Department of Stomatology, Xinqiao Hospital, Third Military Medical University, Chongqing, China;National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China;State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Engineering Research Center of Oral Translational Medicine, Ministry of Education and National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China;

关键词: bone regeneration;    DLP 3D printing;    biphasic calcium phosphate;    polydopamine;    bone morphogenetic protein-2 (BMP-2);   

DOI  :  10.3389/fbioe.2022.854693

来源: DOAJ

【 摘 要 】

Digital light processing (DLP)-based 3D printing is suitable to fabricate bone scaffolds with small size and high precision. However, the published literature mainly deals with the fabrication procedure and parameters of DLP printed bioceramic scaffold, but lacks the subsequent systematic biological evaluations for bone regeneration application. In this work, a biphasic calcium phosphate (BCP) macroporous scaffold was constructed by DLP-based 3D printing technique. Furthermore, bone morphogenetic protein-2 (BMP-2) was facilely incorporated into this scaffold through a facile polydopamine (PDA) modification process. The resultant scaffold presents an interconnected porous structure with pore size of ∼570 μm, compressive strength (∼3.6 MPa), and the self-assembly Ca-P/PDA nanocoating exhibited excellent sustained-release property for BMP-2. Notably, this BMP-2/PDA-BCP scaffold presents favorable effects on the adhesion, proliferation, osteogenic differentiation, and mineralization of bone marrow stromal cells (BMSCs). Furthermore, in vivo experiments conducted on rats demonstrated that the scaffolds could induce cell layer aggregation adjacent to the scaffolds and continuous new bone generation within the scaffold. Collectively, this work demonstrated that the BMP-2/PDA-BCP scaffold is of immense potential to treat small craniofacial bone defects in demand of high accuracy.

【 授权许可】

Unknown