Molecular Selection Of Tomato And Pepper Breeding Lines Possessing Resistance Alleles Against Tobamoviruses

Agriculture

Mihálik Daniel¹  Kraic Ján¹  Šliková Svetlana¹  Klčová Lenka¹  Hudcovicová Martina¹  Korbelová Erika² 

[1] National Agricultural and Food Center – Research Institute of Plant Production Piešťany;Zelseed spol. s.r.o., Horná Potôň;

关键词: tomato;    pepper;    molecular marker;    tobamovirus;    tm-2;    tm-2;    l3 gene;   

DOI  :  10.1515/agri-2015-0008

来源: DOAJ

【 摘 要 】

Molecular selection among newly created tomato and pepper lines was applied for identification of lines possessing DNA markers linked with the resistant loci against tobamoviruses ToMV, TMV, PaMMV, and PMMoV. Only four tomato lines among 184 had DNA marker linked with the resistant allele Tm-2 conferring homozygosity at this locus. Resistance of these four lines was tested and confirmed also by virological testing by inoculation with TMV strain 0. Simultaneously tested lines heterozygous at this locus expressed full or unbalanced resistance. Fifty-eight out of 62 tested pepper lines had only marker linked to susceptible allele of the locus L. Three lines derived from tobamovirus resistant pepper cultivars Brill and Brilliant expressed marker linked to resistant allele L3, and only one line derived from resistant cultivar Hurricane possessed both markers. Four selected pepper lines declared resistance also after artificial inoculation with the TMV P0 pathotype. Molecular selection, both in tomato and pepper breeding lines, may be useful in breeding programs directed to tobamovirus resistance.

【 授权许可】

Unknown