Towards a Novel Patch Material for Cardiac Applications: Tissue-Specific Extracellular Matrix Introduces Essential Key Features to Decellularized Amniotic Membrane

International Journal of Molecular Sciences

Volkmar Falk¹  Christof Stamm²  Aarón X. Herrera Martin²  Matthias Becker²  Martina Seifert²  Maria Schneider²  Oliver Klein²  Janita A. Maring²  Thorsten Braun³ 

[1] Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 13353 Berlin, Germany;Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, Germany;Department of Obstetrics and Gynecology, Charite Medical University, 13353 Berlin, Germany;

关键词: extracellular matrix;    hydrogel;    cardioprotection;    patch;    epicardium;    amnion;    immunocompatibility;   

DOI  :  10.3390/ijms19041032

来源: DOAJ

【 摘 要 】

There is a growing need for scaffold material with tissue-specific bioactivity for use in regenerative medicine, tissue engineering, and for surgical repair of structural defects. We developed a novel composite biomaterial by processing human cardiac extracellular matrix (ECM) into a hydrogel and combining it with cell-free amniotic membrane via a dry-coating procedure. Cardiac biocompatibility and immunogenicity were tested in vitro using human cardiac fibroblasts, epicardial progenitor cells, murine HL-1 cells, and human immune cells derived from buffy coat. Processing of the ECM preserved important matrix proteins as demonstrated by mass spectrometry. ECM coating did not alter the mechanical characteristics of decellularized amniotic membrane but did cause a clear increase in adhesion capacity, cell proliferation and viability. Activated monocytes secreted less pro-inflammatory cytokines, and both macrophage polarization towards the pro-inflammatory M1 type and T cell proliferation were prevented. We conclude that the incorporation of human cardiac ECM hydrogel shifts and enhances the bioactivity of decellularized amniotic membrane, facilitating its use in future cardiac applications.

【 授权许可】

Unknown