Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro

BMC Veterinary Research

Maria Cekanova¹  Dawn U. Hickey¹  Sony Pandey¹  Marti Drum¹  Darryl L. Millis¹ 

[1] Department of Small Animal Clinical Siences, College of Veterinary Medicine, The University of Tennessee;

关键词: Canine;    Cell viability;    Platelet-rich plasma;    Osteoarthritis;    Regenerative medicine;    Multipotent mesenchymal stromal cells;   

DOI  :  10.1186/s12917-019-2010-x

来源: DOAJ

【 摘 要 】

Abstract Background Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay. Results Positive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs. Conclusion PRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.

【 授权许可】

Unknown