A Novel Porcine Graft for Regeneration of Bone Defects

Materials

Haw-Ming Huang¹  Wei-Fang Lee²  Eisner Salamanca³  Chin-Yi Lin³  Sheng-Wei Feng³  Wei-Jen Chang³  Che-Tong Lin³ 

[1] Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine,Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan;School of Dental Technology, College of Oral Medicine, Taipei Medical University,250 Wu-Hsing Street, Taipei 110, Taiwan;School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan;

关键词: bone regeneration;    bone graft;    porcine tissue;    in vivo test;    hydroxyapatite;   

DOI  :  10.3390/ma8052523

来源: DOAJ

【 摘 要 】

Bone regeneration procedures require alternative graft biomaterials tothose for autogenous bone. Therefore, we developed a novel porcine graftusing particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarialbone defects and compared the graft properties with those of commercialhydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks.Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-sizedefects of 5 mm diameter and 3 mm depth were prepared. The defects werefilled with HA/β-TCP,250–500 μm or 500–1000 μm porcine graft, andcontrol defects were not filled.The animals were grouped for sacrificeat 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks wereprepared for micro-computed tomography (micro-CT) scanning andhistological sectioning. Similar bone formations were observed in allthree treatment groups, although the 250–500 μm porcine graft performedslightly better. Rabbit calvarial bone tissue positively responded toporcine grafts and commercial HA/β-TCP, structural analyses showedsimilar crystallinity and porosity of the porcine and HA/β-TCP grafts,which facilitated bone formation through osteoconduction. These porcinegrafts can be considered as graft substitutes, although furtherdevelopment is required for clinical applications.

【 授权许可】

Unknown