Thidiazuron induced plant regeneration in callus culture of triploid banana (Musa sp.) ‘Gros Michel’, AAA group

Songklanakarin Journal of Science and Technology (SJST)

关键词: banana;    callus culture;    ‘Gros Michel’ AAA group;    organogenesis;    thidiazuron;   

DOI  :  

来源: DOAJ

【 摘 要 】

Yellow friable calluses of banana (Musa sp.) ‘Gros Michel’, AAA group were initiated from in vitro shoot buds cultured on Murashige and Skoog (MS) solid medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and coconut water (CW). Small spherical, compact calluses were formed after friable calluses were transferred to half-MS liquid media supplemented with 1.5 mg/l 2, 4-D or 1.5 mg/l 2, 4-D in combination with 5% CW or without 2,4-D and CW. However, no shoots were produced from these media. Embryogenic calluses were induced followed by subculture the spherical, compact calluses to half-MS solid medium in the presence of thidiazuron (TDZ). These embryogenic calluses gave rise to shoots on MS germination medium containing 2.0 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzyladenine (BA). Complete banana plants were recovered on MS maturation medium containing 0.5 mg/l BA and 0.2% activated charcoal. Morphological observation of plantlets did not show any abnormality.

【 授权许可】

Unknown