Substituted Pyrazinecarboxamides as Abiotic Elicitors of Flavolignan Production in Silybum marianum (L.) Gaertn Cultures in Vitro

Molecules

Lenka Tůmová¹  Jiří Tůma¹  Klara Megušar¹ 

[1] ¹Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic ²Department of Biology, Faculty of Education, University of Hradec Králové, Víta Nejedlého 573, 500 03 Hradec Králové, Czech Republic

关键词: pyrazinecarboxamides;    abiotic elicitors;    milk thistle (Silybum marianum L.);    silymarin;    flavonolignan production;    callus culture;   

DOI  :  10.3390/molecules15010331

来源: mdpi

【 摘 要 】

Substituted pyrazinecarboxamides markedly influenced production of flavonolignans in Silybum marianum callus and suspension cultures. In this study the effect of two compounds, N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide (1) and N-(3-iodo-4-methylphenyl)-5-tert-butyl-pyrazine-2-carboxamide (2), as abiotic elicitors on flavono-lignan production in callus culture of S. marianum was investigated. Silymarin complex compounds have hepatoprotective, anticancer and also hypocholesterolemic activity. In vitro flavonolignan concentration in cells is very low and the elicitation is one of the methods to increase production. Elicitors were tested at three concentrations and at different culture times. In the case of elicitation with 1, the greatest increase of flavonolignan and taxifoline production was observed at concentration c_1a after 6-hours of elicitation and after 24 and 72-hours at concentration c_1b. However, increased production of silychristin, one of the compounds in the silymarin complex, was achieved after only 6-hours elicitation with c_1a (2.95 × 10^-4 mol/L). The content of silychristin was 2-times higher compared to the control sample. An increased production of silychristin was reached with compound 2 at the concentration c₂ (2.53 × 10^-3 mol/L) after 72 h of elicitation. The production of silychristin in this case was increased 12-times compared to control.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.