Parasites & Vectors | |
Molecular epidemiological studies on animal trypanosomiases in Ghana | |
关键词: Trypanosomiasis; Human African Trypanosomiasis; Ghana; PCR; | |
DOI : 10.1186/1756-3305-5-217 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
African trypanosomes are extracellular protozoan parasites that are transmitted between mammalian hosts by the bite of an infected tsetse fly. Human African Trypanosomiasis (HAT) or sleeping sickness is caused by
Methods
A total of 219 tsetse flies, 248 pigs and 146 cattle blood samples were collected from Adidome and Koforidua regions in Ghana in 2010. Initial PCR assays were conducted using the internal transcribed spacer one (ITS1) of ribosomal DNA (rDNA) primers, which can detect most of the pathogenic trypanosome species and
Results
The overall prevalence of trypanosomes were 17.4% (38/219), 57.5% (84/146) and 28.6% (71/248) in tsetse flies, cattle and pigs, respectively.
Conclusion
Our results showed that there is a high prevalence of parasites in both tsetse flies and livestock in the study areas in Ghana. This enhances the need to strengthen control policies and institute measures that help prevent the spread of the parasites.
【 授权许可】
Unknown