Mixed phytochemicals mediated synthesis of copper nanoparticles for anticancer and larvicidal applications

Heliyon

Pavadai Parasuraman¹  Madasamy Sundar²  Sakkanan Ilango³  Ambikapathi Nivetha⁴  Sattanathan Kumar⁵  Theivendran Panneerselvam⁶  Sankaranarayanan Murugesan⁷  Gopalan Rajagopal⁸  Selvaraj Kunjiappan⁸ 

[1] Science Pilani, Pilani Campus, Vidya Vihar, Pilani, 333031, Rajasthan, India;Centre for Research and Postgraduate Studies in Botany, Ayya Nadar Janaki Ammal College, Sivakasi, 626124, Tamil Nadu, India;Deparment of Pharmaceutical Chemistry, Paavai College of Pharmacy and Research, Namakkal, 637018, Tamil Nadu, India;Department of Chemistry, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, M S R Nagar, Bengaluru, 560054, Karnataka, India;Department of Pharmaceutical Chemistry, Swamy Vivekanadha College of Pharmacy, Elayampalayam, Tiruchengodu, 637205, Tamil Nadu, India;;Department of Pharmacy, Birla Institute of Technology &Postgraduate and Research Department of Zoology, Ayya Nadar Janaki Ammal College, Sivakasi, 626124, Tamil Nadu, India;

关键词: Wrightia tinctoria R.Br;    GC-MS;    Copper nanoparticles;    Anticancer activity;    Larvicidal activity;    Molecular docking and hemolytic assay;   

DOI  :  

来源: DOAJ

【 摘 要 】

The synthesis of copper nanoparticles (CuNPs) using Wrightia tinctoria (Wt) R.Br extract is defined in this article as being convenient, environmentally friendly, and non-toxic. UV-visible spectrophotometry, FT-IR, XRD, particle size analyser, SEM-EDAX and TEM methods were used to describe the physicochemical properties of Wt extract mediated synthesized CuNPs (Wt-CuNPs). The Wt-CuNPs synthesized was found to be monodispersed and spherical, with an average size of 15 nm. Gas chromatography and mass spectrometry (GC-MS) research revealed that the Wt R.Br plant extract contains various phytochemical compounds. The properties of Wt-CuNPs were verified by the findings of characterization tests. Via in silico molecular docking experiments with established targets, the underlying mechanisms of cytotoxicity against breast cancer and larvicidal behaviour against Aedes aegypti of Wt-CuNPs were investigated. Interestingly, in vitro cytotoxicity studies showed 50% cell death (IC50) of Wt-CuNPs treated MCF-7 cells and Vero Cells (Kidney epithelial cells) were displayed at 119.23 μg.mL−1 and 898.75 μg.mL−1, respectively. Also, Wt-CuNPs showed least LC50 and LC90 values for larvicidal activity against A. aegypti were of 32.10 μg.mL−1 and 21.70 μg.mL−1, respectively. Furthermore, Wt-CuNPs is found to be less toxic and biocompatible in haemolytic assays. The findings clearly showed that biosynthesized Wt-CuNPs have been used as a possible anticancer and larvicidal agent, as well as being environmentally friendly.

【 授权许可】

Unknown