Stress Resistance of Saccharomyces cerevisiae Strains Overexpressing Yeast Polyphosphatases

Stresses

Nadeshda Andreeva¹  Lubov Ryazanova¹  Larisa Ledova¹  Ludmila Trilisenko¹  Tatiana Kulakovskaya¹ 

[1] Federal Research Center, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Skryabin Institute of Biochemistry and Physiology of Microorganisms, 142290 Pushchino, Russia;

关键词: polyphosphate;    polyphosphatase;    yeast;    oxidative stress;    manganese stress;    alkali stress;   

DOI  :  10.3390/stresses2010002

来源: DOAJ

【 摘 要 】

Inorganic polyphosphate (polyP) is an important factor in the stress resistance of microorganisms. The polyphosphate-overexpressing strains of yeast S. cerevisiae were used as a model for studying the inter-relationship between stress resistance and polyP level. We compared the polyP level and resistance to the oxidative, manganese, cadmium, and alkaline stresses in parent stain CRN and in strains overexpressing the four yeast polyphosphatases: Ppx1, Ppn1, Ppn2, and Ddp1. Strains overexpressing Ppx1, Ppn1, and Ppn2 have lower polyP content and the strain overexpressing Ddp1 has the same polyP content as the parent strain. The strains overexpressing Ppn1 and Ddp1 show higher resistance to peroxide and manganese. The strain overexpressing Ppx1 showed a decrease in peroxide resistance. The strain overexpressing Ppn2 was more resistant to alkaline and peroxide stresses. A similar increase in resistance to the manganese and peroxide stresses of strains overexpressing Ppn1 and Ddp1, which differ in polyP content, led to the conclusion that there is no direct relationship between polyP content and variations in this resistance. Thus, we speculate about the potential role of inositol pyrophosphates as signaling molecules in stress response.

【 授权许可】

Unknown