Proteins required for vacuolar function are targets of lysine polyphosphorylation in yeast

FEBS Letters

article

Liam McCarthy¹  Amanda Bentley-DeSousa¹  Alix Denoncourt¹  Yi-Chieh Tseng¹  Matthew Gabriel¹  Michael Downey¹ 

[1] Department of Cellular and Molecular Medicine, University of Ottawa;Ottawa Institute of Systems Biology, University of Ottawa

关键词: Apl5;    polyP;    polyphosphate;    polyphosphorylation;    Prb1;    vacuole;    yeast;   

DOI  :  10.1002/1873-3468.13588

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of polyP. In this study, we evaluated the polyphosphorylation status of over 200 candidate targets in Saccharomyces cerevisiae. We report eight new polyphosphorylated proteins that interact genetically and physically with previous targets implicated in ribosome biogenesis. The expanded target network includes vacuolar proteins Prb1 and Apl5, whose modification with polyP suggests a model for feedback regulation of polyP synthesis, while raising questions regarding the location of polyphosphorylation in vivo.

【 授权许可】

Unknown