Differential association between S100A4 levels and insulin resistance in prepubertal children and adult subjects with clinically severe obesity

Obesity Science & Practice

Laura Campderros¹  Francesc Villarroya¹  Rocío Puig²  David Sánchez‐Infantes²  Eva Martínez²  Silvia Pellitero²  Manel Puig‐Domingo²  Siri D. Taxerås²  Irene Piquer‐Garcia²  Icíar Lucena²  Marta Murillo³  Joan Bel³  María Martínez³  Jordi Tarascó⁴  Pau Moreno⁴  Marta Ramon‐Krauel⁵  Carles Lerin⁵  María Galán⁶  José Balibrea⁷ 

[1] Department of Biochemistry and Molecular Biology, Institute of Biomedicine University of Barcelona Barcelona Spain;Department of Endocrinology and Nutrition Germans Trias i Pujol Research Institute Barcelona Spain;Department of Pediatric Germans Trias i Pujol Research Institute Barcelona Spain;Department of Surgery Germans Trias i Pujol Research Institute Barcelona Spain;Endocrinology Department Institut de Recerca Sant Joan de Déu Barcelona Spain;Institut de Recerca del Hospital de la Santa Creu i Sant Pau‐Programa ICCC Barcelona Spain;Metabolic and Bariatric Surgery Unit, EAC‐BS Center of Excellence Vall d'Hebron University Hospital Barcelona Spain;

关键词: insulin resistance;    obesity;    prepubertal children;    S100A4;   

DOI  :  10.1002/osp4.381

来源: DOAJ

【 摘 要 】

Summary Objectives S100A4 has been recently identified as an adipokine associated with insulin resistance (IR) in adult subjects with obesity. However, no data about its levels in children with obesity and only a few approaches regarding its potential mechanism of action have been reported. To obtain a deeper understanding of the role of S100A4 in obesity, (a) S100A4 levels were measured in prepubertal children and adult subjects with and without obesity and studied the relationship with IR and (b) the effects of S100A4 in cultured human adipocytes and vascular smooth muscle cells (VSMCs) were determined. Methods Sixty‐five children (50 with obesity, age 9.0 ±1.1 years and 15 normal weight, age 8.4 ±0.8 years) and fifty‐nine adults (43 with severe obesity, age 46 ±11 years and 16 normal weight, age 45 ±9 years) were included. Blood from children and adults and adipose tissue samples from adults were obtained and analysed. Human adipocytes and VSMC were incubated with S100A4 to evaluate their response to this adipokine. Results Circulating S100A4 levels were increased in both children (P = .002) and adults (P < .001) with obesity compared with their normal‐weight controls. In subjects with obesity, S100A4 levels were associated with homeostatic model assessment‐insulin resistance (HOMA‐IR) in adults (βstd = .42, P = .008) but not in children (βstd = .12, P = .356). Human adipocytes were not sensitive to S100A4, while incubation with this adipokine significantly reduced inflammatory markers in VSMC. Conclusions Our human data demonstrate that higher S100A4 levels are a marker of IR in adults with obesity but not in prepubertal children. Furthermore, the in vitro results suggest that S100A4 might exert an anti‐inflammatory effect. Further studies will be necessary to determine whether S100A4 can be a therapeutic target for obesity.

【 授权许可】

Unknown