Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain

Plants

Eugenia Cerda-Castillo¹  Lorena Carro¹  Raúl Rivas¹  JoséDavid Flores-Félix¹  Encarna Velázquez¹  Andrea Squartini² 

[1] Departamento de Microbiologíay Genética, Universidad de Salamanca, 37007 Salamanca, Spain;Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, Italy;

关键词: pea;    Pisum sativum;    Rhizobium laguerreae;    symbiosis;    quorum sensing;    nodulation effectiveness;   

DOI  :  10.3390/plants9121755

来源: DOAJ

【 摘 要 】

Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide.

【 授权许可】

Unknown