Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought

Phytobiomes Journal

Ian Hodge¹  Daniel A. Jacobson²  Piet Jones²  Gerald A. Tuskan³  Timothy J. Tschaplinski³  Sara Jawdy³  Benjamin J. Garcia³  Jessy L. Labbé³  Lee Gunter³  Xiaohan Yang³  Paul E. Abraham⁴  Sharlee Climer⁵ 

[1] Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831 and Department of Computer Science, Stanford University, Stanford, CA 94305;Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831 and The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville 37996;Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831;Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831;Department of Mathematics and Computer Science, University of Missouri, St. Louis 63121;

关键词: bacteriology;    microbiome;    mutualism;    mycology;    nematology;    nutrient cycling;    rhizosphere and phyllosphere;    symbiosis;    transcriptomics;    Populus deltoides;   

DOI  :  10.1094/PBIOMES-04-18-0021-R

来源: DOAJ

【 摘 要 】

Plant drought stress causes systematic changes to photosynthesis, metabolism, growth, and potentially the phytobiome. Additionally, drought affects plants in both a species-specific and water-deficit-driven manner, causing the response to drought to be dependent both on how drought is being experienced and on any adaptation to prior drought exposure. Thus, understanding the effect of drought on plants requires assessing drought response in multiple conditions, such as progressive acute drought and recurrent cyclic drought, and at different levels of severity. In this study, we have utilized RNA sequencing to identify changes to the plant transcriptome and the phytobiome during both acute progressive drought and cyclic drought at multiple severities. Co-analysis of the plant and phytobiome, utilizing the same RNAseq data, allows for the identification of novel associations that would not be possible otherwise. We have identified that the drought response ranges from increased transcripts related to photosynthesis and metabolic activity in mild acute drought to decreased transcripts related to photosynthesis and metabolic impairment in severe drought. Moreover, while water deficit is a main driver of transcriptional responses in severe drought, there are increases in reactive oxygen species (ROS) metabolism and photosynthetic transcripts in cyclic severe drought compared with acute severe drought, independent of water deficit. The phytobiome exhibits alternate responses to drought when compared with the transcriptome. Specifically, the phytobiome is affected more by the cyclic or acute nature of the drought rather than the severity of the drought, with the phytobiome having an increase in taxa under cyclic drought that are often reported to have beneficial effects on the plants. Lastly, we have identified associations between taxa in the phytobiome with expression of disease response, ROS metabolism, and photosynthesis transcripts suggesting interplay between the host plant and its phytobiome in response to drought.

【 授权许可】

Unknown