Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts

Cells

Jong-Keun Seon¹  SookJung Yun¹  Sandra Franz²  RubénA. Ferrer²  Jan-Christoph Simon²  Marie Schneider³  Hanluo Li⁴  Tina Kottek⁴  Bernd Lethaus⁴  Vuk Savković⁴  Kensuke Yamauchi⁵  Danilo Obradović⁶  Sebastian Hahnel⁷  FedericaFrancesca Masieri⁸ 

[1] Chonnam National University Hwasun Hospital, Hwasun-gun 58128, Korea;Clinic for Dermatology, Venereology and Allergology, University Hospital Leipzig, 04103 Leipzig, Germany;Clinic for Hematology, Cell Therapy and Hemostaseology, University Hospital Leipzig, 04103 Leipzig, Germany;Department of Cranial Maxillofacial Plastic Surgery, University Clinic Leipzig, 04103 Leipzig, Germany;Kensuke Yamauchi, Department of Oral & Maxillofacial Surgery, Tohoku University, Sendai 980-8577, Japan;Leipzig Heart Center, 04289 Leipzig, Germany;Polyclinic for Dental Prosthetics and Material Sciences, University Hospital Leipzig, 04103 Leipzig, Germany;School of (EAST) Engineering, Arts, Science & Technology, University of Suffolk, IP41QJ Ipswich, UK;

关键词: mesenchymal stem cells;    hair follicle;    regenerative medicine;    non-invasive;    cell therapy;    adipose tissue;   

DOI  :  10.3390/cells9092069

来源: DOAJ

【 摘 要 】

Background: Regenerative therapies based on autologous mesenchymal stem cells (MSC) as well as stem cells in general are still facing an unmet need for non-invasive sampling, availability, and scalability. The only known adult source of autologous MSCs permanently available with no pain, discomfort, or infection risk is the outer root sheath of the hair follicle (ORS). Methods: This study presents a non-invasively-based method for isolating and expanding MSCs from the ORS (MSCORS) by means of cell migration and expansion in air–liquid culture. Results: The method yielded 5 million cells of pure MSCORS cultured in 35 days, thereby superseding prior art methods of culturing MSCs from hair follicles. MSCORS features corresponded to the International Society for Cell Therapy characterization panel for MSCs: adherence to plastic, proliferation, colony forming, expression of MSC-markers, and adipo-, osteo-, and chondro-differentiation capacity. Additionally, MSCORS displayed facilitated random-oriented migration and high proliferation, pronounced marker expression, extended endothelial and smooth muscle differentiation capacity, as well as a paracrine immunomodulatory effect on monocytes. MSCORS matched or even exceeded control adipose-derived MSCs in most of the assessed qualities. Conclusions: MSCORS qualify for a variety of autologous regenerative treatments of chronic disorders and prophylactic cryopreservation for purposes of acute treatments in personalized medicine.

【 授权许可】

Unknown