A strategy design based on antibiotic‑resistance and plasmid replicons genes of clinical Escherichia coli strains

Bioengineered

Junyan Liu¹  Ling Yang²  Dingqiang Chen³  Thanapop Soteyome⁴  Yanrui Ye⁵  Xin Lin⁶  Zhenbo Xu⁶ 

[1] College of Light Industry and Food Science, Guangdong Provincial Key Laboratoryof Lingnan Specialty Food Science and Technology, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China;Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou University, Guangzhou Medical University, Guangzhou, Guangdong, China;Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China;Home Economics Technology, Rajamangala University of Technology Phra Nakhon, Bangkok, Thailand;School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China;School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, China;

关键词: AMR;    β-lactam;    plasmid replicon;    antimicrobial susceptibility;    ESBLs;    molecular epidemiology;   

DOI  :  10.1080/21655979.2022.2047543

来源: DOAJ

【 摘 要 】

Since antimicrobial resistance, especially β-lactam resistance genes were common in clinical Escherichia coli strains, this study had designed and developed multiplex amplification platform for rapid and accurate detection of such resistance genes in 542 clinical E. coli isolates. The obtained specimens were subjected to bacteriological examination, antimicrobial susceptibility testing, and detection of β-lactamase genes and plasmid replicons. The major virulence genes were detected by 7 groups of multiplex PCR and eight groups of multiplex PCR were designed to detect 8 different plasmid replicons including parA-parB, iteron, repA, and RNAI. It was found that most MDR isolates were co-resistant to penicillins (AMP) and fluoroquindones (LVX, CIP) and distribution of LVX and CIP resistance was significantly higher among female than male gender. RNAI (AY234375) showed the highest detection rate, followed by the iteron (J01724) and repA (M26308), indicating the relatively higher carriage rate of corresponding plasmids. BlaOXA acquired the highest carriage rate, followed by group 2 blaCTX-M and blaSHV-1, indicating their prevalence among clinical E. coli. Among the β-lactamase genes, blaOXA acquired the highest carriage rate, followed by group 2 blaCTX-M and blaSHV-1, indicating their prevalence among clinical E. coli. The RNAI (AY234375) showed the highest detection rate, followed by the iteron (J01724) and repA (M26308), indicating the relatively higher carriage rate of the corresponding plasmids by clinical E. coli isolates. It is shown that the developed multiplex amplification methodology is applicable to AMR detection, and such identification of plasmid replicons and β-lactamase genes may aid in the understanding of clinical E. coli isolate epidemiology.

【 授权许可】

Unknown