Therapeutic Effects of Dipterocarpus tuberculatus with High Antioxidative Activity Against UV-Induced Photoaging of NHDF Cells and Nude Mice

Antioxidants

YunJu Choi¹  DaeYoun Hwang¹  SoHae Park¹  SuJin Lee¹  JeongEun Gong¹  JiEun Kim¹  JuMin Park²  HeeSeob Lee²  Bae-Hwan Kim³  Onevilay Souliya⁴  Bounleuane Douangdeuane⁴ 

[1] Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Longevity & Wellbeing Research Center, Pusan National University, Miryang 50463, Korea;Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Korea;Department of Public Health, Keimyung University, Daegu 42601, Korea;Institute of Traditional Medicine, Ministry of Health, Vientiane 0103, Lao PDR;

关键词: Dipterocarpus tuberculatus;    UV;    photoaging;    antioxidants;    skin aging;   

DOI  :  10.3390/antiox10050791

来源: DOAJ

【 摘 要 】

To investigate the therapeutic effects of methanol extracts of Dipterocarpus tuberculatus Roxb. (MED) against UV-induced photoaging, we assessed for alterations in the antioxidant activity, anti-apoptotic effects, ECM modulation, skin appearances, and anti-inflammatory response in normal human dermal fibroblast (NHDF) cells and nude mice orally treated with MED. High levels of tannin content and high free radical scavenging activity to DPPH were determined in MED, while seven active components, namely, gallic acid, bergenin, ellagic acid, ε-viniferin, asiatic acid, oleanolic acid, and 2α-hydroxyursolic acid, were identified using LC–MS analyses. UV-induced alterations in the NO concentration, SOD activity, and Nrf2 expression were remarkably recovered in MED-treated NHDF cells. Moreover, the decreased number of apoptotic cells and G2/M phase arrest were observed in the UV + MED-treated groups. Similar recoveries were detected for β-galactosidase, MMP-2/9 expression, and intracellular elastase activity. Furthermore, MED treatment induced suppression of the COX-2-induced iNOS mediated pathway, expression of inflammatory cytokines, and inflammasome activation in UV-radiated NHDF cells. The anti-photoaging effects observed in NHDF cells were subsequently evaluated and validated in UV + MED-treated nude mice through skin phenotypes and histopathological structure analyses. Taken together, these results indicate that MED exerts therapeutic effects against UV-induced photoaging and has the potential for future development as a treatment for photoaging.

【 授权许可】

Unknown