Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches

Biotechnology for Biofuels

André Ferraz¹  Daniele Sporck¹  Felipe A. M. Reinoso¹  Adriane M. F. Milagres¹  Ana Gutiérrez²  Jorge Rencoret²  José C. del Rio² 

[1] Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo;Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC;

关键词: Xylan;    Sugarcane;    Biorefinery;    Cellulose hydrolysis;    Alkaline-sulfite pretreatment;   

DOI  :  10.1186/s13068-017-0981-z

来源: DOAJ

【 摘 要 】

Abstract Background New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis of the glucan component is a way to add value to the hemicellulose fraction, which can be used in papermaking, pharmaceutical, and food industries. Hemicellulose removal can also facilitate subsequent cellulolytic glucan hydrolysis. Results Sugarcane bagasse was pretreated with an alkaline-sulfite chemithermomechanical process to facilitate subsequent extraction of xylan by enzymatic or alkaline procedures. Alkaline extraction methods yielded 53% (w/w) xylan recovery. The enzymatic approach provided a limited yield of 22% (w/w) but produced the xylan with the lowest contamination with lignin and glucan components. All extracted xylans presented arabinosyl side groups and absence of acetylation. 2D-NMR data suggested the presence of O-methyl-glucuronic acid and p-coumarates only in enzymatically extracted xylan. Xylans isolated using the enzymatic approach resulted in products with molecular weights (Mw) lower than 6 kDa. Higher Mw values were detected in the alkali-isolated xylans. Alkaline extraction of xylan provided a glucan-enriched solid readily hydrolysable with low cellulase loads, generating hydrolysates with a high glucose/xylose ratio. Conclusions Hemicellulose removal before enzymatic hydrolysis of the cellulosic fraction proved to be an efficient manner to add value to sugarcane bagasse biorefining. Xylans with varied yield, purity, and structure can be obtained according to the extraction method. Enzymatic extraction procedures produce high-purity xylans at low yield, whereas alkaline extraction methods provided higher xylan yields with more lignin and glucan contamination. When xylan extraction is performed with alkaline methods, the residual glucan-enriched solid seems suitable for glucose production employing low cellulase loadings.

【 授权许可】

Unknown