Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Materials

Elena Mavropoulos¹  Carlos Fernando de Almeida Barros Mourão²  Vinicius Schott Gameiro²  Pietro Montemezzi²  Gutemberg Gomes Alves²  Joice Correa²  Angelo Cardarelli³  Suzana Azevedo dos Anjos⁴  Mariana Rodrigues Pereira⁵  Victor Hugo de Souza⁶  Ana Carolina Batista Brochado⁶ 

[1] Brazilian Center for Physics Research, Rio de Janeiro 22290-180, Brazil;Clinical Research Unit, Antônio Pedro University Hospital, Fluminense Federal University, Niteroi 24033-900, Brazil;Department of Dentistry, University Vita Salute San Raffaele, 20100 Milan, Italy;National Institute for Metrology, Standardization and Metrological Quality, Xerém 25250-020, Brazil;Neurobiology Department, Institute of Biology, Fluminense Federal University, Niteroi 24210-201, Brazil;Post-Graduation Program in Science & Biotechnology, Institute of Biology, Fluminense Federal University, Niteroi 24210-201, Brazil;

关键词: tridimensional cell culture;    osteoconductivity;    biocompatibility;    bone;    biomaterials;    titanium;   

DOI  :  10.3390/ma14195858

来源: DOAJ

【 摘 要 】

Successful biomaterials for bone tissue therapy must present different biocompatible properties, such as the ability to stimulate the migration and proliferation of osteogenic cells on the implantable surface, to increase attachment and avoid the risks of implant movement after surgery. The present work investigates the applicability of a three-dimensional (3D) model of bone cells (osteospheres) in the evaluation of osteoconductive properties of different implant surfaces. Three different titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA). The surfaces were characterized by Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM), confirming that they present very distinct roughness. After seeding the osteospheres, cell–surface interactions were studied in relation to cell proliferation, migration, and spreading. The results show that BE surfaces present higher densities of cells, leaving the aggregates towards than titanium surfaces, providing more evidence of migration. The PSHA surface presented the lowest performance in all analyses. The results indicate that the 3D model allows the focal analysis of an in vitro cell/surfaces interaction of cells and surfaces. Moreover, by demonstrating the agreement with the clinical data observed in the literature, they suggest a potential use as a predictive preclinical tool for investigating osteoconductive properties of novel biomaterials for bone therapy.

【 授权许可】

Unknown