Identification of key biomarkers in steroid-induced osteonecrosis of the femoral head and their correlation with immune infiltration by bioinformatics analysis

BMC Musculoskeletal Disorders

Yu Su¹  Jun Zhao²  Junjie Guan²  Xingshi Zhang²  Jizhao Jiang² 

[1] Harbin Fifth Hospital;Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine;

关键词: Steroid-induced osteonecrosis of the femoral head;    Diagnostic;    Immune cells;    CIBERSORT;    Bioinformatics analysis;   

DOI  :  10.1186/s12891-022-04994-7

来源: DOAJ

【 摘 要 】

Abstract Objective This study aimed to identify key diagnostic markers and immune infiltration of (SONFH) by bioinformatics analysis. Methods Related SONFH datasets were downloaded from the Gene Expression Omnibus (GEO) database. First, we identified the differentially expressed genes (DEGs) and performed the functional enrichment analysis. Then weighted correlation network analysis (WGCNA) and the MCODE plug-in in Cytoscape were used to identify the diagnostic markers of SONFH. Finally, CIBERSORT was used to analyze the immune infiltration between SONFH and healthy controls, and the correlation between infiltrating immune cells and diagnostic markers was analyzed. Results TYROBP, TLR2, P2RY13, TLR8, HCK, MNDA, and NCF2 may be key diagnostic markers of SONFH. Immune cell infiltration analysis revealed that Memory B cells and activated dendritic cells may be related to the SONFH process. Moreover, HCK was negatively correlated with CD8 T cells, and neutrophils were positively correlated with those key diagnostic markers. Conclusions TYROBP, TLR2, P2RY13, TLR8, HCK, MNDA, and NCF2 may be used as diagnostic markers of SONFH, and immune-related mechanism of SONFH and the potential immunotherapy are worthy of further study.

【 授权许可】

Unknown