Hypoxic acclimation improves mitochondrial bioenergetic function in large yellow croaker Larimichthys crocea under Cu stress

Ecotoxicology and Environmental Safety

Bing Hu¹  Chun-Xiang Ai²  Ping Cao³  Wei Song⁴  Hui Zhang⁵  Lin Zeng⁵  Wen-Cheng Li⁵ 

[1] Corresponding author.;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, PR China;East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, PR China;Fujian Province Key Laboratory of Special Aquatic Formula Feed, Fuqing 350300, PR China;National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China;

关键词: Hypoxic acclimation;    Mitochondria;    Oxidative phosphorylation;    Cu exposure;   

DOI  :  

来源: DOAJ

【 摘 要 】

The purpose of this study was to investigate how pre-hypoxia exposure affected the mitochondrial structure and bioenergetic function of large yellow croaker in responding to Cu stress. Fish were acclimated to normoxia and 3.0 mg DO L−1 for 48 h, then subjected to 0 and 120 μg Cu L−1 for another 48 h. Hypoxic acclimation did not affect mitochondrial ultrastructure and reactive oxygen species (ROS), but reduced oxidative phosphorylation (OXPHOS) efficiency. Cu exposure impaired mitochondrial ultrastructure, increased ROS generation and inhibited OXPHOS efficiency. Compared with Cu exposure alone, hypoxic acclimation plus Cu exposure reduced ROS production and improved OXPHOS efficiency by enhancing mitochondrial respiratory control ratio, mitochondrial membrane potential, and activities and gene expressions of electron transport chain enzymes. In conclusion, hypoxic acclimation improved the mitochondrial energy metabolism of large yellow croaker under Cu stress, facilitating our understanding of the molecular mechanisms regarding adaptive responses of hypoxia-acclimated fish under Cu stress.

【 授权许可】

Unknown