Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS

Toxins

Yosuke Matsuo¹  Masayo Kushiro¹  Hiroyuki Nakagawa¹  Yuki Sago¹  Hitoshi Nagashima¹  Kentaro Takahara² 

[1] National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan;Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan;

关键词: LC-Orbitrap MS;    fumonisin;    Fusarium;    corn;    N-(1-deoxy-d-fructos-1-yl) fumonisin;   

DOI  :  10.3390/toxins7093700

来源: DOAJ

【 摘 要 】

The existence of glucose conjugates of fumonisin B2 (FB2) and fumonisin B3 (FB3) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB2 and FB3 bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B2 (NDfrc-FB2) and N-(1-deoxy-d-fructos-1-yl) fumonisin B3 (NDfrc-FB3) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB2 and NDfrc-FB3 with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB2 and FB3 molecules occurred to their primary amine residues.

【 授权许可】

Unknown