Detection of Type A Trichothecene Di-Glucosides Produced in Corn by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry

Toxins

Hiroyuki Nakagawa²  Shigeru Sakamoto¹  Yuki Sago² 

[1] ThermoFisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama 221-0022, Japan; E-Mail:;National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan; E-Mails:

关键词: masked mycotoxin;    mycotoxin glucoside;    LC-Orbitrap MS;    trichothecene;    Fusarium;    corn;   

DOI  :  10.3390/toxins5030590

来源: mdpi

【 摘 要 】

The existence of di-glucosylated derivative of T-2 toxin in plant (corn powder) was confirmed for the first time in addition to that of HT-2 toxin. These masked mycotoxins (mycotoxin glucosides) were identified as T-2 toxin-di-glucoside (T2GlcGlc) and HT-2 toxin-di-glucoside (HT2GlcGlc) based on accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometric (LC-Orbitrap MS) analysis. Although the absolute structure of T2GlcGlc was not clarified, two glucose molecules were suggested to be conjugated at 3-OH position in tandem when considering the structure of T-2 toxin. On the other hand, the specification of the structure seems to be more complicated in the case of HT2GlcGlc, since HT-2 toxin has two possible positions (at 3-OH and 4-OH) to be glusocylated. In addition, 15-monoacetoxyscirpenol-glucoside (MASGlc) was also detected in the identical sample.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.