Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

STAR Protocols

Kim D. Falkenberg¹  Magdalena Parys¹  Mieke Dewerchin¹  Rongyuan Chen¹  Xuri Li¹  Elda Meta¹  Liliana Sokol¹  Peter Carmeliet¹  Katerina Rohlenova¹  Mila Borri¹  Koen Veys¹  Melissa García-Caballero²  Nadine V. Conchinha³  Laura P.M.H. de Rooij³  Charlotte Dubois³  Pauline de Zeeuw³  Sébastien J. Dumas³  Lucas Treps³  Laure-Anne Teuwen³  Guy Eelen³  Luc Schoonjans³  Vincent Geldhof³  Joanna Kalucka³  Jermaine Goveia⁴ 

[1] Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium;Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium and Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium;;Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, &State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China;

关键词: Cell isolation;    Flow Cytometry/Mass Cytometry;    Single Cell;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Endothelial cells (ECs) harbor distinct phenotypical and functional characteristics depending on their tissue localization and contribute to brain, eye, lung, and muscle diseases such as dementia, macular degeneration, pulmonary hypertension, and sarcopenia. To study their function, isolation of pure ECs in high quantities is crucial. Here, we describe protocols for rapid and reproducible blood vessel EC purification established for scRNA sequencing from murine tissues using mechanical and enzymatic digestion followed by magnetic and fluorescence-activated cell sorting.For complete details on the use and execution of these protocol, please refer to Kalucka et al. (2020), Rohlenova et al. (2020), and Goveia et al. (2020).

【 授权许可】

Unknown