Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver

STAR Protocols

Nadine V. Conchinha¹  Charlotte Dubois¹  Mieke Dewerchin¹  Jermaine Goveia¹  Xuri Li¹  Pauline de Zeeuw¹  Elda Meta¹  Lucas Treps¹  Peter Carmeliet¹  Vincent Geldhof¹  Koen Veys¹  Magdalena Parys²  Kim D. Falkenberg³  Laura P.M.H. de Rooij³  Rongyuan Chen³  Melissa García-Caballero³  Sébastien J. Dumas³  Liliana Sokol³  Laure-Anne Teuwen³  Guy Eelen³  Luc Schoonjans³  Mila Borri³  Joanna Kalucka³  Katerina Rohlenova⁴ 

[1] Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium;Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium and Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium;;Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, &State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China;

关键词: Cell isolation;    Single Cell;    Flow Cytometry/Mass Cytometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues.For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).

【 授权许可】

Unknown