Analysis of Phenolic Content in Grape Seeds and Skins by Means of a Bio-Electronic Tongue

Sensors

GuilhermeGobbi Teixeira¹  LuísGuimaraes Dias¹  Fernando Martin-Pedrosa²  Cristina Garcia-Cabezon²  MariaLuz Rodriguez-Mendez³  Coral Salvo-Comino³  Celia García-Hernandez³ 

[1] Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, 5301 Bragança, Portugal;Department of Materials Science, Universidad de Valladolid, 47011 Valladolid, Spain;Group UVaSens, Escuela de Ingenierías Industriales, Universidad de Valladolid, Paseo del Cauce 59, 47011 Valladolid, Spain;

关键词: electrochemical sensor;    bio-electronic tongue;    grape seed;    grape skin;    phenol;   

DOI  :  10.3390/s20154176

来源: DOAJ

【 摘 要 】

A bio-electronic tongue has been developed to evaluate the phenolic content of grape residues (seeds and skins) in a fast and easy way with industrial use in mind. A voltammetric electronic tongue has been designed based on carbon resin electrodes modified with tyrosinase combined with electron mediators. The presence of the phenoloxydase promotes the selectivity and specificity towards phenols. The results of multivariate analysis allowed discriminating seeds and skins according to their polyphenolic content. Partial least squares (PLS) has been used to establish regression models with parameters related to phenolic content measured by spectroscopic methods i.e., total poliphenol content (TPC) and Folin–Ciocalteu (FC) indexes. It has been shown that electronic tongue can be successfully used to predict parameters of interest with high correlation coefficients (higher than 0.99 in both calibration and prediction) and low residual errors. These values can even be improved using genetic algorithms for multivalent analysis. In this way, a fast and simple tool is available for the evaluation of these values. This advantage may be due to the fact that the electrochemical signals are directly related to the phenolic content.

【 授权许可】

Unknown