Genetic Polymorphisms of the TGFB1 Signal Peptide and Promoter Region: Role in Wilms Tumor Susceptibility?

Journal of Kidney Cancer and VHL

Carolina Batista Ariza¹  Marla Karine Amarante²  Glauco Akelinghton Freire Vitiello²  Maria Angelica Ehara Watanabe²  Carlos Eduardo Coral de Oliveira²  Cintya Mayumi Ishibashi²  Bruna Karina Banin Hirata²  Karen Brajão de Oliveira²  Roberta Losi Guembarovski²  Marina Okuyama Kishima³  Alda Losi Guembarovski⁴ 

[1] Department of Biology, State University of Londrina, Parana, Brazil.;Department of Pathological Sciences, State University of Londrina, Parana, Brazil.;Department of Pathology, Clinical and Toxicological Analysis, Health Science Center, State University ofLondrina, Londrina, Parana, Brazil.;Department of Pathology, Clinical and Toxicological Analysis, Health Science Center, State University of Londrina, Londrina, Parana, Brazil.;

关键词: genetic polymorphism;    nephroblastoma;    prognosis;    susceptibility;    TGFB1;    Wilms tumor;   

DOI  :  10.15586/jkcvhl.v8i4.182

来源: DOAJ

【 摘 要 】

The aim of the present study was to investigate the rs1800468 (G-800A), rs1800469 (C-509T), rs1800470 (C29T), and rs1800471 (G74C) TGFB1 genetic polymorphisms and their haplotype structures in patients with Wilms Tumor (WT) and neoplasia-free controls. The genomic DNA was extracted from 35 WT patients and 160 neoplasia-free children, and the TGFB1 polymorphisms were genotyped by polymerase chain reaction, followed by restriction fragment length polymorphism. The haplotype structures were inferred, and permutation and logistic regression tests were performed to check for differences in haplotype distribution between the control and WT individuals. Positive associations were found in the recessive model for rs1800469 T allele (OR: 8.417; 95% CI: 3.177 to 22.297; P < 0.001) and for the rs1800470 C allele (OR: 3.000; 95% CI: 1.296 to 6.944; P = 0.01). Haplotype analysis revealed a significant negative association between GCTG and WT (OR: 0.236, 95% CI: 0.105 to 0.534; P = 0.0002); by contrast, the GTTG haplotype was associated with increased risk for WT (OR: 12.0; 95% CI: 4.202 to 34.270; P < 0.001). Furthermore, rs1800469 was negatively correlated with tumor size and a trend toward a positive correlation for capsular invasion was observed in the dominant model (Tau-b: −0.43, P = 0.02 and tau-b: 0.5, P = 0.06, respectively). This is the first study with rs1800468, rs1800469, rs1800470, and rs1800471 TGFB1 polymorphisms in WT, and our results suggest that the TGFB1 promoter and signal peptide region polymorphisms may be associated with WT susceptibility and clinical presentation.

【 授权许可】

Unknown