Semi-Quantification of Lectins in Rice (Oryza sativa L.) Genotypes via Hemagglutination

Agronomy

Sumaira Naz¹  Muhammad Zahoor¹  Hidayat Hussain²  Muhammad Asif Nawaz³  Haseena Gulzar³  Essam A. Ali⁴  Riaz Ullah⁵  Farhat Ali Khan⁶  Dil Naz⁷  Asad Jan⁸ 

[1] Department of Biochemistry, University of Malakand, Chakdara Dir Lower, Malakand 18800, Khyber Pakhtunkhwa, Pakistan;Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Salle), Germany;Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Peshawar 18000, Khyber Pakhtunkhwa, Pakistan;Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Peshawar 18000, Khyber Pakhtunkhwa, Pakistan;Department of Zoology, University of Malakand, Chakdara Dir Lower, Malakand 18800, Khyber Pakhtunkhwa, Pakistan;Institute of Biotechnology and Genetic Engineering, Agriculture University Peshawar, Peshawar 18000, Khyber Pakhtunkhwa, Pakistan;

关键词: rice;    Oryza sativa L.;    lectins;    hemagglutination assay;    rabbit RBCs;    titer values;   

DOI  :  10.3390/agronomy11101899

来源: DOAJ

【 摘 要 】

Lectins are unique glycoproteins that react with specific sugar residues on cell surfaces resulting in agglutination. They offer enormous applications in therapeutics, diagnostics, medicine, and agriculture. Rice lectins are naturally expressed during biotic and abiotic stresses suggesting their importance in stress resistance physiology. The objective of this study was to determine the presence and relative concentration of lectins in different accessions of rice obtained from IABGR/NARC Islamabad mainly originated from Pakistan. About 210 rice accessions including 02 local varieties and 05 transgenic seeds were screened for seed lectins using a hemagglutination (HA) assay with 5% Californian bred rabbits’ erythrocytes. A protein concentration of 3–8 mg/100 mg of seed flour was measured for all the rice accessions; the highest was 8.03 mg for accession 7600, while the lowest noted was 3.05 mg for accession 7753. Out of 210 accessions, 106 showed the highest HA activity. These 106 genotypes were further screened for titer analysis and specific activity. The highest titer and specific activity were observed for accession 7271 as 1024 and 236 hemagglutination unit (HAU), respectively. The selected accessions’ relative affinity and HA capability were evaluated using blood from four different sources: human, broiler chicken, local rabbit, and Californian-breed rabbit. The highest HA activity was observed with Californian-breed rabbit RBCs. The lectin assay was stable for about 1–2 h. After the required investigations, the accessions with higher lectin concentration and HA capability could be used as a readily available source of lectins for further characterization and utilization in crop improvement programs.

【 授权许可】

Unknown