Synergistic Effect of rhBMP-2 Protein and Nanotextured Titanium Alloy Surface to Improve Osteogenic Implant Properties

Metals

Yadir Torres¹  José Antonio Rodríguez-Ortiz¹  Andrea Mesa-Restrepo²  Norman Balcázar³  Juan José Pavón⁴  Robinson Montes⁴  Juan Fernando Alzate⁵  Edwin Patiño⁶  Ana Civantos⁷  Jean Paul Allain⁷ 

[1] Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41003 Sevilla, Spain;Department of Biomedical Engineering, Pennsylvania State University, State College, PA 16802, USA;Department of Physiology and Biochemistry, School of Medicine, University of Antioquia, Medellin 50022, Colombia;Grupo de Biomateriales Avanzados y Medicina Regenerativa BAMR, Facultad de Ingeniería, Universidad de Antioquia, Medellín 50022, Colombia;National Center for Genomic Sequencing-CNSG, School of Medicine, University of Antioquia, Medellin 50022, Colombia;Structural Biochemistry of Macromolecules, Chemistry Institute, University of Antioquia, Medellin 50022, Colombia;The Ken and Mary Alice Lindquist Department of Nuclear Engineering, Pennsylvania State University, State College, PA 16802, USA;

关键词: surface modification;    bone morphogenetic proteins;    directed irradiation synthesis;    nanopatterning;    advanced biointerfaces;    osseointegration;   

DOI  :  10.3390/met11030464

来源: DOAJ

【 摘 要 】

One of the major limitations during titanium (Ti) implant osseointegration is the poor cellular interactions at the biointerface. In the present study, the combined effect of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) and nanopatterned Ti6Al4V fabricated with Directed irradiation synthesis (DIS) is investigated in vitro. This environmentally-friendly plasma uses ions to create self-organized nanostructures on the surfaces. Nanocones (≈36.7 nm in DIS 80°) and thinner nanowalls (≈16.5 nm in DIS 60°) were fabricated depending on DIS incidence angle and observed via scanning electron microscopy. All samples have a similar crystalline structure and wettability, except for sandblasted/acid-etched (SLA) and acid-etched/anodized (Anodized) samples which are more hydrophilic. Biological results revealed that the viability and adhesion properties (vinculin expression and cell spreading) of DIS 80° with BMP-2 were similar to those polished with BMP-2, yet we observed more filopodia on DIS 80° (≈39 filopodia/cell) compared to the other samples (<30 filopodia/cell). BMP-2 increased alkaline phosphatase activity in all samples, tending to be higher in DIS 80°. Moreover, in the mineralization studies, DIS 80° with BMP-2 and Anodized with BMP-2 increased the formation of calcium deposits (>3.3 fold) compared to polished with BMP-2. Hence, this study shows there is a synergistic effect of BMP-2 and DIS surface modification in improving Ti biological properties which could be applied to Ti bone implants to treat bone disease.

【 授权许可】

Unknown