Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles

Nanomaterials

Saurabh Dixit¹  ShreeR. Singh¹  David Martin¹  Elijah Nyairo¹  DieudonnéR. Baganizi²  SkylaA. Duncan²  Rajnish Sahu²  VidaA. Dennis²  Francois Villinger² 

[1] Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA;;Center for NanoBiotechnology &

关键词: chlamydia;    recombinant major outer membrane protein (rMOMP);    PLA-PEG nanoparticles;    IL-10;    cytokines;    SOCS1;    SOCS3;    inflammation;    anti-inflammatory;    therapy;   

DOI  :  10.3390/nano9081074

来源: DOAJ

【 摘 要 】

Inflammation, as induced by the presence of cytokines and chemokines, is an integral part of chlamydial infections. The anti-inflammatory cytokine, interleukin (IL)-10, has been reported to efficiently suppress the secretion of inflammatory cytokines triggered by Chlamydia in mouse macrophages. Though IL-10 is employed in clinical applications, its therapeutic usage is limited due to its short half-life. Here, we document the successful encapsulation of IL-10 within the biodegradable polymeric nanoparticles of PLA-PEG (Poly (lactic acid)-Poly (ethylene glycol), to prolong its half-life. Our results show the encapsulated-IL-10 size (~238 nm), zeta potential (−14.2 mV), polydispersity index (0.256), encapsulation efficiency (~77%), and a prolonged slow release pattern up to 60 days. Temperature stability of encapsulated-IL-10 was favorable, demonstrating a heat capacity of up to 89 °C as shown by differential scanning calorimetry analysis. Encapsulated-IL-10 modulated the release of IL-6 and IL-12p40 in stimulated macrophages in a time- and concentration-dependent fashion, and differentially induced SOCS1 and SOCS3 as induced by chlamydial stimulants in macrophages. Our finding offers the tremendous potential for encapsulated-IL-10 not only for chlamydial inflammatory diseases but also biomedical therapeutic applications.

【 授权许可】

Unknown